牦牛卵母细胞及体外受精胚胎早期发育基因差异表达的研究

doi:10.11843/j.issn.0366-6964.2013.10.010

Abstract

Abstract:

The purpose of this study was to clarify differential pattern of mRNA expression for genes related to different developmental stages of yak oocytes and preimplatation embryos, and to provide molecular evidence for gene expression of preimplatation embryonic development. The mRNA differential display reverse transcription PCR was used to identify differential expression bands for sequencing and alignment. The levels of mRNA relative expression were evaluated by RT-PCR in oocyte, 2-cell embryo, 8-cell embryo and blastocyst in yak. The results showed that three differential expression bands were highly homologous with zinc finger MYND-type containing 11 (ZMYND11), ribosomal protein L27a (RPL27A) and nucleosome assembly protein 1-like 1 (NAP1L1), respectively, except that one gene during morula stage showed no homology to any other known genes in GenBank. The relative expression levels of ZMYND11 genes increased in matured oocyte, 2-cell embryo, 8-cell embryo and in the blastocyst, with no significant differences between these stages (P>0.05). The relative expression levels of NAP1L1 genes were relatively low in matured oocyte, 2-cell embryo and blastocyst, with sharp increase in 8-cell embryo (P<0.05). The relative expression levels of RPL27A genes decreased during development, from matured oocyte to 2-cell, then to 8-cell embryo, and increased significantly in the blastocyst (P<0.05). ZMYND11, RPL27A and NAP1L1, the mRNA expression levels of which showed differences in different development stages of yak oocytes and preimplantation embryos, might be related to normal development and different physiological activities of preimplantation embryo in yak.

CLC Number:

GUO Xian, DING Xue-zhi, WANG Hong-bo, BAO Peng-jia, YAN Ping. Gene Expression of Yak Oocytes and Preimplantation Embryos Derived from in vitro Fertilization[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(10): 1576-1581.

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Gene Expression of Yak Oocytes and Preimplantation Embryos Derived from in vitro Fertilization

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